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Network Member Profile

Current Field of Study

• Developmental fate and lineage differentiation of somatic and germ cells during embryogenesis
• Morphogenesis and pattern formation during gastrulation and organogenesis
• Transcriptional regulation and craniofacial development of normal and mutant embryos
• Phenotypic analysis of mouse embryos with mutations of type II procollagen (Col2a1) gene
• Differentiation and organogenesis of endodermal tissues
• Developmental genetics and pathogenesis of Rett Syndrome using mouse models
• Molecular genetics of congenital defects of the anterior segment of the eye

Australian Collaborators

• A/Prof John Christodoulou, Western Sydney Genetics Program, Children's Hospital at Westmead, NSW
• Dr Sally Dunwoodie, Victor Chang Cardiac Research Institute, NSW
• Professor Andrew Boyd, Queensland Institute of Medical Research, QLD
• Professor Michael Waters, Institute of Molecular Biosciences, QLD
• Dr Roger Reddel, Children's Medical Research Institute, NSW
• Dr Lorraine Robb, Walter and Eliza Hall Institute for Medical Research

International Collaborators

• Professor Richard Behringer (University of Texas, USA)
• Dr Heiner Westphal (National Institute of Child Health and Development, Bethesda, USA)
• Dr Andras Nagy (Samuel Lunenfeld Research Institute, Toronto, Canada)
• Professor Kathy Cheah (University of Hong Kong)
• Professor Huda Zoghbi (Baylor College of Medicine, Houston, Texas, USA)
• Drs Masami Kanai-Azuma and Yoshiakira Kanai (University of Tokyo, Japan)
• Dr Masato Ota, Tokyo Medical and Dental University, Japan

Facilities

• Transgenesis and Gene Targeting
• Mass Spectrometry for proteomic analysis
• Confocal Microscope (with environmental control)
• Fluorescence microscope equipped with digital imaging capability
• High throughput robotic whole mount in situ hybridization system
• Micromanipulation system for cells to whole mouse embryos
• BioServices Unit with Importation / Quarantine facility
• Genetic and embryological models for analyzing the cellular and molecular mechanisms of the establishment of the developmental blueprint for morphogenesis and organ formation
• Micromanipulation and imaging of postimplantation mouse embryos maintained in whole-embryo culture

Recent Publications

Yamaguchi YL, Tanaka SS, Kasa M, Yasuda K, Tam PPL and Matsui Y (2006) Expression of low density lipoprotein receptor-related protein 4 (Lrp4) gene in the mouse germ cells. Gene Expression Patterns 6:607-612

Duffy SL, Steiner KA, Tam PPL and Boyd AW (2006) Expression analysis of the Epha1 Receptor Tyrosine Kinase and its high-affinity ligands Efna1 and Efna3 during early mouse development. Gene Expression Patterns 6(7):719-23

Pelka GJ, Watson CM, Radziewic T, Hayward M, Christodoulou J and Tam PPL (2006) Mecp2 deficiency is associated with learning and cognitive deficits and altered gene activity in the hippocampal region of mice. Brain 129, 887-898

Willcock C, Grigg J, Wilson M, Tam P Billson F and Jamieson R (2006) Congenital iris ectropion as an indicator of variant aniridia. British Journal of Ophthmology 90(5):658-659

Yamaguchi YL, Tanaka SS, Yasuda K, Matsui Y and Tam PPL (2006) Stage-specific Importin13 activity influences meiosis of germ cells in the mouse. Developmental Biology 297, 350-360 doi:10.1016/j.ydbio.2006.04.465

Tanaka S S, Yamaguchi Y L, Tsoi B, Lickert H and Tam P P L (2005) IFITM/mil/fragilis family proteins, IFITM1 and IFITM3, play distinct roles in mouse primordial germ cell homing and repulsion. Developmental Cell 9:745-756

Watson CM, Pelka G, Radziewic T, Shahbazian MD, Christodoulou J, Williamson SL, Tam PP. (2005) Reduced proportion of Purkinje cells expressing paternally derived mutant Mecp2308 allele in female mouse cerebellum is not due to a skewed primary pattern of X-inactivation. Hum Mol Genet. 2005 May 11; [Epub ahead of print]

Loebel D A F , Tsoi B, Wong N and Tam P P L (2005) A conserved non-coding intronic transcript at the mouse Dnm3 locus. Genomics

Pelka G J, Watson C M, Christodoulou J and Tam P P L (2005) Distinct expression profiles of Mecp2 transcripts with different lengths of 3’UTR in the brain and visceral organs during mouse development. Genomics 85(4):441-52

Weaving L S, Christodoulou J, Williamson S L, Friend K L , McKenzie O L D, Archer H, Evans J, Clarke A, Pelka G J, Tam P P L, Watson C, Lahooti H, Ellaway C J, Bennetts B, Leonard H and Gécz J (2004)

Mutations of CDKL5 cause a severe neurodevelopmental disorder with infantile spasms and mental retardation

American Journal of Human Genetics 75(6):1079-1093

Robb L and Tam P P L (2004) Gastrula organizer and embryonic patterning in the mouse Seminars in Cell and Developmental Biology 15(5):543-554

Rossant J and Tam P P L (2004) Emerging asymmetries and embryonic patterning in early mouse development. Developmental Cell 7:155-164

Loebel D A F, Tsoi B, Wong N, O’Rourke M P and Tam P P L (2004) Restricted expression of ETn-related sequences during post-implantation mouse development Gene Expression Patterns 4:467-471

Ota M S, Loebel D A F, O’Rourke M P, Wong N, Tsoi B, and Tam P P L (2004) Twist is required for patterning the cranial nerves and maintaining the viability of mesodermal cells. Developmental Dynamics 230:216-228

Ding H, Schertzer M, Wu X, Gertsenstein M, Kammori M, Pourvali R, Poon S, Vulto I, Chavez E, Tam P P L, Nagy A and Lansdorp P M (2004) Regulation of Murine Telomere Length by Rtel: An Essential Gene Encoding Helicase-like Protein Cell 117(7):873-886

Tanaka S S, Nagamatsu G, Tokitake Y, Miyuki Kasa M, Tam P P L and Matsui Y (2004) Regulation of expression of mouse interferon-induced transmembrane protein like gene-3, Ifitm3 (mil-1, fragilis), in germ cells Developmental Dynamics 230:651-659

Tam P P L, Khoo P-L, Wong N, Tsang T E and Behringer R R (2004) Regionalization of cell fates and cell movement in the endoderm of the mouse gastrula and the impact on loss of Lhx1 ( Lim1) function Developmental Biology 274 :171-187

Ding H, Wu Xi, Boström H, Kim I, Koh G Y, Wong N, Tsoi B, O’Rourke M, Soriano P, Betsholtz C, Hart T C, Marazita M L, Field L L, Tam P P L, and Nagy A. (2004) ­A specific requirement for PDGF-C in palate formation and PDGFR-a signaling. Nature Genetics 36:1112-1116

Davidson B P, Tsang T E, Khoo P-L, Gad J M and Tam P P L (2003) Introduction of cell markers into germ layer tissues of the mouse gastrula by whole embryo electroporation. Genesis 35(1), 57-62

Hukriede N A, Tsang T E, Habas R, Poh-Lynn Khoo P L, Steiner K, Weeks D L, Tam P P L and Dawid I B (2003) Conserved requirement of Lim1 function for cell movements during gastrulation. Developmental Cell 4, 83-94

Tam P P L, Kanai-Azuma M and Kanai Y (2003) Early endoderm development in vertebrates: Lineage differentiation and morphogenetic function. Current Opinion in Genetics and Development 13, 393-400.

Loebel D A F, Watson C M, De Young A and Tam P P L (2003) Lineage choice and differentiation in mouse embryos and embryonic stem cells. Developmental Biology 264, 1-14

Tam P P L and Rossant J (2003) Mouse embryonic chimeras: Tools for studying mammalian development. Development 130, 6155-6163